Single sperm cell isolation by micromanipulation for human identification in sexual assault

This study proposes a new alternative solution in preferential extraction methods or microdissection to isolate and analyse single sperm cells in case of sexual assault. After the transfer of swabs in liquid culture medium, perpetrator's spermatozoas can be physically separated from victim's epithelial cells by using classical techniques of micromanipulation as ICSI (IntraCytoplasmic Spermatozoa Injection), usually applied for IVF (In vitro Fertilization).     

单个细胞DNA样本的制备及其在灵敏度分析中的应用研究

目的建立一种用于高精确灵敏度分析的微量DNA样本制备方法。方法用显微捕获技术制备以细胞为单位的DNA样本,并用于ProfilerPlus试剂盒及ABI310遗传分析仪的灵敏度测试。结果建立了单细胞捕获操作方案,成功制备了依次含1～11个细胞的一组DNA分析样本,并可准确用于ProfilerPlus誖试剂盒及ABI310遗传分析仪的灵敏度研究。结论基于显微操作技术的高精确微量DNA分析样本制备方法可准确用于分析技术方法的灵敏度研究     

苓桂术甘汤对免疫功能低下模型小鼠淋巴细胞活性的影响

目的:观察苓桂术甘汤对免疫功能低下模型小鼠免疫功能的影响.方法:近交系昆明种小鼠以环磷酰胺(Cy,80 mg/kg,ip,qd×1)诱导免疫功能低下,用苓桂术甘汤(42.90,21.45,4.29 g/kg)连续灌胃(ig)给药7 d,分别采用免疫溶血法、四氮唑盐比色法(MTT)和小鼠胸腺细胞氚标胸腺嘧啶核苷(3H-TdR)掺入法测定血清溶血素、NK细胞及IL-2活性.结果:苓桂术甘汤能明显促进Cy所致免疫功能低下模型小鼠血清溶血素生成,增强NK细胞及IL-2活性,与模型组比较,差异有显著性(P＜0.05或P＜0.01).结论:苓桂术甘汤对Cy所致免疫功能低下模型小鼠三类淋巴细胞的免疫活性均具有明显的激活作用.     

校园手机短信文化的调查报告

通过对大学生手机消费情况、手机短信功能的调查与分析,阐述了手机短信文化的消极和积极作用.从中让我们发现,虽然短信文化是现代社会兴起的一种流行文化,但是如何规范手机短信也是当务之急.     

海南鳽胃肠道内分泌细胞的免疫组织化学定位及形态观察

运用组织学、免疫组织化学方法对海南的胃肠道内分泌细胞分布、内分泌细胞的免疫组织化学定位及形态特点进行了初步研究。发现海南的胃及小肠各段都有嗜银细胞,其中胃和前肠分布最多,中肠、后肠逐渐减少。银染细胞在胃组织中以圆形和不规则形的居多,在小肠段以长棒状、圆形的居多。应用生长抑素抗血清、胰高血糖素抗血清、胃泌素抗血清对海南胃肠道内分泌细胞进行免疫组织化学定位,发现生长抑素细胞在胃肠道均有分布,其中在腺胃和前肠最多,往后逐渐减少;在胃肠道均未发现胃泌素细胞和胰高血糖素细胞。     

Sudden unexpected death due to a previously undiagnosed plasma cell dyscrasia

The plasma cell dyscrasias are a diverse group of disorders characterized by the production of a clonal paraprotein. Sudden death is a recognized complication of the plasma cell dyscrasias, most commonly in individuals with cardiac involvement by amyloidosis. However, the current forensic literature has no reported cases in which sudden death resulted from complications of a plasma cell dyscrasia that was first diagnosed by postmortem histologic examination. We present the case of a woman whose sudden and unexpected death resulted from a seizure. Postmortem examination revealed no evidence of trauma or a grossly identifiable natural disease process that would have accounted for her death. However, microscopic and immunohistologic studies revealed a previously undiagnosed plasma cell dyscrasia, the clonality of which was determined by immunohistochemical studies for immunoglobulin light chains, that was not associated with amyloid deposition. This case elucidates a previously unrecognized cause of sudden unexpected death and illustrates the importance of microscopic studies in selected cases examined in medical examiner/coroner offices.     

Brokers and Entrepreneurs in Human Stem Cell Governance Networks in the United States

The policy debate over embryonic stem cell (ESC) research in the United States mainly focuses on the extent to which the government ought to provide resources for that research. This article addresses the strategic use of network positions to mobilize an effective coordination system for lifting the moratorium on funding of ESC under the Obama administration since March 2009. By identifying patterns of interactions between members in a network of embedded ties, we can explore the major actors who play the role of brokers or entrepreneurs in stem cell research funding policy under the Obama administration. For that, this article utilizes social network analysis and the G&F brokerage roles framework to identify key brokers in facilitating coordination among various members engaged in funding human stem cell research in the governance network.     

沙冬青生物碱对MDCC-MSB1细胞增殖抑制与诱导凋亡的影响

采用体外细胞培养技术、噻唑蓝(MTT)还原法、透射电子显微镜观察及流式细胞技术检测梯度浓度沙冬青总生物碱对体外培养鸡马立克氏病肿瘤HDCC-MSB1细胞的增殖抑制及诱导凋亡的作用。结果显示,沙冬青生物碱可显著抑制HDCC-MSB1细胞增殖,且这种抑制具有剂量和时间效应关系。经沙冬青生物碱作用的HDCC-MSB1细胞培养样本中有明显的DNA低含量颗粒(亚G1期峰),细胞周期各时相分布发生改变,细胞增殖在G1被阻滞,并进一步诱导HDCC-MSB1细胞凋亡。电子显微镜观察也表明,经沙冬青生物碱作用的HDCC-MSB1细胞培养样本中,大量HDCC-MSB1细胞凋亡。结果表明,沙冬青生物碱对HDCC-MSB1细胞增殖有显著抑制效应,其抑制机理为诱导HDCC-MSB1细胞凋亡。     

化痰通瘀解毒方提取物对人胃癌细胞SGC-7901侵袭转移能力的影响

目的 观察化痰通瘀解毒方提取物对人胃癌细胞株SGC-7901侵袭转移能力的影响。方法 10、20、40 μg/mL化痰通瘀解毒方提取物分别干预人胃癌细胞SGC-7901 24 h后，采用Transwell小室法检测人胃癌细胞SGC-7901的侵袭、迁移能力，分别采用酶联免疫吸附试验和Western blot法检测SGC-7901细胞中基质金属蛋白酶9（matrix metalloproteinase 9, MMP-9）和E-钙黏蛋白（E-cadherin）的表达水平。结果 4组SGC-7901的侵袭细胞数和迁移细胞数以及E-cadherin和MMP-9表达水平比较，差异均具有统计学意义（P＜0.05），呈现明显的剂量依赖性，以高剂量化痰解毒方提取物的效应最明显。结论 化痰解毒方可抑制人胃癌细胞SGC-7901侵袭和迁移能力，其作用机制可能与上调E-cadherin表达，下调MMP-9的表达有关。     

鼻腔免疫禽流感灭活疫苗对鸡呼吸道肥大细胞分布的影响

用添加CpG的禽流感病毒(H5亚型)灭活疫苗对7日龄罗曼公鸡进行鼻腔免疫,14日龄加强免疫一次;分别于首免后第3、5、7周剖杀,取鼻腔、喉部、气管、气管叉及肺组织,通过甲苯胺蓝染色法显示肥大细胞,观察呼吸道各部位肥大细胞的分布及数量变化。结果显示,首免后第3周气管和气管叉黏膜中的肥大细胞数量显著增加(P<0.05),第5周时喉部和气管叉黏膜中的肥大细胞数量显著增加(P<0.05),气管黏膜中肥大细胞数量也极显著增加(P<0.01),第7周肺中的肥大细胞数量显著增加(P<0.05);肥大细胞的数量在喉部最多,气管叉次之,气管和肺较少。试验结果表明,局部黏膜免疫可增加呼吸道肥大细胞的数量,肥大细胞可能参与黏膜免疫反应。